pBS-V9
(Plasmid
#173797)
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PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBluescript KS (+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 6944
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Vector typeBacterial Expression, Plant Expression
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Selectable markersspectinomycin and Streptomycin (500ug/ml)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameaadA
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Alt nameStreptomycin and spectinomycin-resistance
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Speciesbacteria
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Insert Size (bp)1151
Gene/Insert 2
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Gene/Insert nameClpP1
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SpeciesSilene noctiflora
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-V9 was a gift from Daniel Sloan (Addgene plasmid # 173797 ; http://n2t.net/addgene:173797 ; RRID:Addgene_173797) -
For your References section:
Rapid sequence evolution is associated with genetic incompatibilities in the plastid Clp complex. Abdel-Ghany SE, LaManna LM, Harroun HT, Maliga P, Sloan DB. Plant Mol Biol. 2022 Feb;108(3):277-287. doi: 10.1007/s11103-022-01241-4. Epub 2022 Jan 17. 10.1007/s11103-022-01241-4 PubMed 35039977