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Addgene

pBS-V4
(Plasmid #173794)

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Item Catalog # Description Quantity Price (USD)
Plasmid 173794 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBluescript KS (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 6760
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Spectinomycin and Streptomycin (500ug/ml)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    aadA
  • Species
    Bacteria
  • Insert Size (bp)
    1151

Gene/Insert 2

  • Gene/Insert name
    ClpP1
  • Species
    Nicotiana tabacum
  • Insert Size (bp)
    933

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-V4 was a gift from Daniel Sloan (Addgene plasmid # 173794 ; http://n2t.net/addgene:173794 ; RRID:Addgene_173794)

  • For your References section:

    Rapid sequence evolution is associated with genetic incompatibilities in the plastid Clp complex. Abdel-Ghany SE, LaManna LM, Harroun HT, Maliga P, Sloan DB. Plant Mol Biol. 2022 Feb;108(3):277-287. doi: 10.1007/s11103-022-01241-4. Epub 2022 Jan 17. 10.1007/s11103-022-01241-4 PubMed 35039977
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