PB-UBC-mARG3
(Plasmid
#173793)
-
PurposeMammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 173793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePB-UBC
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGvpJ, F, G, L, K
- Promoter UBC promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PB-UBC-mARG3 was a gift from Xiaojun Lian (Addgene plasmid # 173793 ; http://n2t.net/addgene:173793 ; RRID:Addgene_173793) -
For your References section:
A drug-selectable acoustic reporter gene system for human cell ultrasound imaging. Howells AR, Welch PJ, Kim J, Forest CR, Shi C, Lian XL. Bioeng Transl Med. 2023 Aug 2;9(2):e10584. doi: 10.1002/btm2.10584. eCollection 2024 Mar. 10.1002/btm2.10584 PubMed 38435822