Skip to main content
Addgene

pE_Cre_lambdaCos
(Plasmid #173785)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pE-FLP
  • Vector type
    Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    CY2120
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cre
  • Species
    Other
  • Mutation
    S102Y and S279Y- please see depositor comments

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.

The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pE_Cre_lambdaCos was a gift from Keith Shearwin (Addgene plasmid # 173785 ; http://n2t.net/addgene:173785 ; RRID:Addgene_173785)

  • For your References section:

    The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli. Hao N, Chen Q, Dodd IB, Shearwin KE. ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215. 10.1021/acssynbio.1c00215 PubMed 34190535
Related items:
Commonly requested with: