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Addgene

pHelper_O
(Plasmid #173783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHelper
  • Backbone manufacturer
    A portion of this plasmid was derived from a plasmid made by Haldimann and Wanner J. Bact 183, 6384-6393 (2001).
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    186 Integrase
  • Species
    Other
  • Mutation
    E59G - please see depositor comments

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms E59G in 186 Integrase does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper_O was a gift from Keith Shearwin (Addgene plasmid # 173783 ; http://n2t.net/addgene:173783 ; RRID:Addgene_173783)

  • For your References section:

    The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli. Hao N, Chen Q, Dodd IB, Shearwin KE. ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215. 10.1021/acssynbio.1c00215 PubMed 34190535
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