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Addgene

HisGB1-FusionL21A + CBP TAZ1(340-439)
(Plasmid #173763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 6258
  • Modifications to backbone
    introduced second RBS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CITED2(216-246L231A):Hif1a(796-826)
  • Alt name
    Hif1a
  • Alt name
    CITED2
  • Species
    H. sapiens (human)
  • Mutation
    fusion of CITED2 and Hif1a
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Entrez Gene
    CITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6GB1 (N terminal on backbone)
    • thrombin (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CBP (340-439)
  • Alt name
    crebbp
  • Alt name
    CBP
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisGB1-FusionL21A + CBP TAZ1(340-439) was a gift from Peter Wright (Addgene plasmid # 173763 ; http://n2t.net/addgene:173763 ; RRID:Addgene_173763)

  • For your References section:

    The molecular basis of allostery in a facilitated dissociation process. Appling FD, Berlow RB, Stanfield RL, Dyson HJ, Wright PE. Structure. 2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 10.1016/j.str.2021.07.011. 10.1016/j.str.2021.07.011 PubMed 34520739
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