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Addgene

CBP TAZ1(340-439)
(Plasmid #173760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21d
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CBP TAZ1
  • Alt name
    CBP CH1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    306
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBP TAZ1(340-439) was a gift from Peter Wright (Addgene plasmid # 173760 ; http://n2t.net/addgene:173760 ; RRID:Addgene_173760)

  • For your References section:

    CBP/p300 TAZ1 domain forms a structured scaffold for ligand binding. De Guzman RN, Wojciak JM, Martinez-Yamout MA, Dyson HJ, Wright PE. Biochemistry. 2005 Jan 18;44(2):490-7. doi: 10.1021/bi048161t. 10.1021/bi048161t PubMed 15641773
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