pET-Sac-Aβ(M1-42/C18C33)
(Plasmid #173759)

• Purpose: Expresses the AβC18C33 peptide.
• Depositing Lab: James Nowick
• Publication: Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264. doi: 10.1021/acs.biochem.1c00739. Epub 2022 Jan 26.

Backbone

• Vector backbone: pET vector
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Aβ(M1-42/C18C33)
• Species: H. sapiens (human)
• Insert Size (bp): 156
• Mutation: changed valine 18 to cysteine; changed glycine 33 to cysteine.
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: Sac1 (not destroyed)
• 5′ sequencing primer: 5'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG TGC -3'
• 3′ sequencing primer: 5'- GTG GGT TCT AAC AAG GGT GCT ATC ATC TGC CTG ATG GTT GGT GGC GTT GTG ATC GCT -3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry