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pET-Sac-Aβ(M1-42/C18C33)
(Plasmid #173759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET vector
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Aβ(M1-42/C18C33)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    156
  • Mutation
    changed valine 18 to cysteine; changed glycine 33 to cysteine.
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site Sac1 (not destroyed)
  • 5′ sequencing primer 5'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG TGC -3'
  • 3′ sequencing primer 5'- GTG GGT TCT AAC AAG GGT GCT ATC ATC TGC CTG ATG GTT GGT GGC GTT GTG ATC GCT -3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Sac-Aβ(M1-42/C18C33) was a gift from James Nowick (Addgene plasmid # 173759 ; http://n2t.net/addgene:173759 ; RRID:Addgene_173759)

  • For your References section:

    A Disulfide-Stabilized Abeta that Forms Dimers but Does Not Form Fibrils. Zhang S, Yoo S, Snyder DT, Katz BB, Henrickson A, Demeler B, Wysocki VH, Kreutzer AG, Nowick JS. Biochemistry. 2022 Feb 15;61(4):252-264. doi: 10.1021/acs.biochem.1c00739. Epub 2022 Jan 26. 10.1021/acs.biochem.1c00739 PubMed 35080857

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