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pDe_Cas9_KANA_R160b
(Plasmid #173757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDe_Cas9
  • Backbone size w/o insert (bp) 15933
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Spectinomycin is only for bacteria and Kanamycin is only for plants.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9_sgRNA1/R160b_sgRNA2/160b
  • gRNA/shRNA sequence
    Unspecified
  • Species
    S. tuberosum Group Phureja DM
  • Promoter AtU6 &StU6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Spectinomycin is only for bacteria and Kanamycin is only for plants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDe_Cas9_KANA_R160b was a gift from Kristina Gruden (Addgene plasmid # 173757 ; http://n2t.net/addgene:173757 ; RRID:Addgene_173757)

  • For your References section:

    CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato. Lukan T, Veillet F, Kriznik M, Coll A, Mahkovec Povalej T, Pogacar K, Stare K, Chauvin L, Chauvin JE, Gruden K. Hortic Res. 2022 Jun 30;9:uhac147. doi: 10.1093/hr/uhac147. eCollection 2022. 10.1093/hr/uhac147 PubMed 36072839
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