pJRK248
(Plasmid
#173755)
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Purposeco-injection plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4762
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Vector typeWorm Expression
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Selectable markersHygromycin ; Roller
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePrps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJRK248 was a gift from Mike Boxem (Addgene plasmid # 173755 ; http://n2t.net/addgene:173755 ; RRID:Addgene_173755) -
For your References section:
CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans. Kroll JR, Remmelzwaal S, Boxem M. Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 10.1093/genetics/iyab163. 10.1093/genetics/iyab163 PubMed 34849800