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Addgene

pJRK137
(Plasmid #173745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMLS257
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 2999
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prps-0::mKate2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3502
  • Promoter Prps-0

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRK137 was a gift from Mike Boxem (Addgene plasmid # 173745 ; http://n2t.net/addgene:173745 ; RRID:Addgene_173745)

  • For your References section:

    CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans. Kroll JR, Remmelzwaal S, Boxem M. Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 10.1093/genetics/iyab163. 10.1093/genetics/iyab163 PubMed 34849800
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