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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS KS(+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnt-3aHA
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Alt namewnt3a
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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Entrez GeneWnt3a (a.k.a. Wnt-3a, vt)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS Wnt-3aHA was a gift from Jan Kitajewski (Addgene plasmid # 17374 ; http://n2t.net/addgene:17374 ; RRID:Addgene_17374) -
For your References section:
Transformation by Wnt family proteins correlates with regulation of beta-catenin. Shimizu H, Julius MA, Giarre M, Zheng Z, Brown AM, Kitajewski J. Cell Growth Differ. 1997 Dec . 8(12):1349-58. PubMed 9419423