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Addgene

pJRK107
(Plasmid #173731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG298
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 2675
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIBN-3xFLAG
  • Alt name
    CIB1 N-terminal region
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    683
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13 (-21) Forward
  • 3′ sequencing primer M13/pUC Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRK107 was a gift from Mike Boxem (Addgene plasmid # 173731 ; http://n2t.net/addgene:173731 ; RRID:Addgene_173731)

  • For your References section:

    CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans. Kroll JR, Remmelzwaal S, Boxem M. Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 10.1093/genetics/iyab163. 10.1093/genetics/iyab163 PubMed 34849800
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