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Addgene

GST-GGA3-VHS-GAT
(Plasmid #173719)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173719 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-5X-1
  • Backbone manufacturer
    Pharmacia Biotech
  • Backbone size w/o insert (bp) 4972
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GGA3
  • Alt name
    golgi associated, gamma adaptin ear containing, ARF binding protein 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    939
  • Entrez Gene
    GGA3
  • Entrez Gene
    GGA2 (a.k.a. VEAR)
  • Entrez Gene
    GGA1
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-GGA3-VHS-GAT was a gift from Juan Bonifacino (Addgene plasmid # 173719 ; http://n2t.net/addgene:173719 ; RRID:Addgene_173719)
  • For your References section:

    GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. Dell'Angelica EC, Puertollano R, Mullins C, Aguilar RC, Vargas JD, Hartnell LM, Bonifacino JS. J Cell Biol. 2000 Apr 3;149(1):81-94. doi: 10.1083/jcb.149.1.81. 10.1083/jcb.149.1.81 PubMed 10747089