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Addgene

p09008_AF1-4+GFP5-7
(Plasmid #173715)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173715 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression ; E. coli cell free protein synthesis extracts

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    hybrid GFP
  • Species
    Synthetic
  • Insert Size (bp)
    711

Gene/Insert 2

  • Gene/Insert name
    mKate2
  • Insert Size (bp)
    699

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p09008_AF1-4+GFP5-7 was a gift from Johannes Kabisch (Addgene plasmid # 173715 ; http://n2t.net/addgene:173715 ; RRID:Addgene_173715)
  • For your References section:

    HyperXpress: Rapid Single Vessel DNA Assembly and Protein Production in Microliterscale. Zibulski DL, Schlichting N, Kabisch J. Front Bioeng Biotechnol. 2022 Apr 1;10:832176. doi: 10.3389/fbioe.2022.832176. eCollection 2022. 10.3389/fbioe.2022.832176 PubMed 35433646