p09008_AF1-4+GFP5-7
(Plasmid
#173715)
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PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression ; E. coli cell free protein synthesis extracts
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namehybrid GFP
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SpeciesSynthetic
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Insert Size (bp)711
Gene/Insert 2
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Gene/Insert namemKate2
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Insert Size (bp)699
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p09008_AF1-4+GFP5-7 was a gift from Johannes Kabisch (Addgene plasmid # 173715 ; http://n2t.net/addgene:173715 ; RRID:Addgene_173715) -
For your References section:
HyperXpress: Rapid Single Vessel DNA Assembly and Protein Production in Microliterscale. Zibulski DL, Schlichting N, Kabisch J. Front Bioeng Biotechnol. 2022 Apr 1;10:832176. doi: 10.3389/fbioe.2022.832176. eCollection 2022. 10.3389/fbioe.2022.832176 PubMed 35433646