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Addgene

pDisCoTune
(Plasmid #173713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisCoTune
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    0.5 % Glucose
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ERV1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ERV1 (a.k.a. YGR029W)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    PDI
  • Alt name
    protein disulfide-isomerase
  • Species
    H. sapiens (human)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisCoTune was a gift from Morten Norholm (Addgene plasmid # 173713 ; http://n2t.net/addgene:173713 ; RRID:Addgene_173713)

  • For your References section:

    DisCoTune: versatile auxiliary plasmids for the production of disulphide-containing proteins and peptides in the E. coli T7 system. Bertelsen AB, Hackney CM, Bayer CN, Kjelgaard LD, Rennig M, Christensen B, Sorensen ES, Safavi-Hemami H, Wulff T, Ellgaard L, Norholm MHH. Microb Biotechnol. 2021 Nov;14(6):2566-2580. doi: 10.1111/1751-7915.13895. Epub 2021 Aug 18. 10.1111/1751-7915.13895 PubMed 34405535
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