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Arp2/3_pBIG2abcd
(Plasmid #173683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG2abcd
  • Backbone size w/o insert (bp) 5668
  • Total vector size (bp) 16996
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Arp2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1185
  • Promoter polyhedrin

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Arp2_seq_for: ATGGATTCGCAGGGCCG
  • 3′ sequencing primer Arp2_seq_rev: TTATCTTACAGTAACACCGAGTTTTTCGAG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Arp3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1254
  • Promoter polyhedrin

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Arp3_seq_for: ATGGCGGGACGCTTGC
  • 3′ sequencing primer Arp3_seq_rev: TTAGGACATGACGCCAAACACG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    ArpC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Promoter polyhedrin

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer -
  • 3′ sequencing primer ArpC2_seq_rev: TTAGCGTGAGGAGAAAGTTTTGCC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    ArpC3-FLAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    594
  • Promoter polyhedrin

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    ArpC4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    504
  • Promoter polyhedrin

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer -
  • 3′ sequencing primer ArpC4_seq_rev: TTAAAAATTTTTCAGGAACTCTTCGGCAAC
    • (Common Sequencing Primers)

Gene/Insert 6

  • Gene/Insert name
    ArpC5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    456
  • Promoter polyhedrin

Cloning Information for Gene/Insert 6

Gene/Insert 7

  • Gene/Insert name
    ArpC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1116
  • Promoter polyhedrin

Cloning Information for Gene/Insert 7

  • Cloning method Gibson Cloning
  • 5′ sequencing primer -
  • 3′ sequencing primer ArpC1_seq_rev: TTATTTAATTTTCAAATCTTTGAGGGCTGATTCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Arp2/3_pBIG2abcd was a gift from Roberto Dominguez (Addgene plasmid # 173683 ; http://n2t.net/addgene:173683 ; RRID:Addgene_173683)

  • For your References section:

    Cryo-EM structure of NPF-bound human Arp2/3 complex and activation mechanism. Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R. Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaaz7651. doi: 10.1126/sciadv.aaz7651. Print 2020 Jun. 10.1126/sciadv.aaz7651 PubMed 32917641
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