pDSAY-vasaCas9sv40
(Plasmid #173669)

• Purpose: Cas9 expression vector for Anopheles
• Depositing Lab: Eric Marois
• Publication: Marois lab kit for CRISPR and transgenesis in Anopheles and Aedes (unpublished)

Backbone

• Vector backbone: pDSAY
• Backbone manufacturer: Marois lab
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 10745
• Modifications to backbone: added a codon-optimised Cas9 gene subcloned from Zhang lab's pX330 and placed under the control of the Anopheles gambiae vasa promoter and a SV40 terminator
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 under control of vasa promoter and SV40 terminator
• Species: Synthetic
• Insert Size (bp): 6754
• Promoter: vasa

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13F
• 3′ sequencing primer: CCGCCGTGACCGTCGA

Resource Information

• Supplemental Documents:
  • pDSAY-vasaCas9sv40.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid can be used to create Cas9-expressing transgenic mosquito lines using the PhiC31 attB / attP system. It can also be used as a helper plasmid to express Cas9 transiently in embryo microinjection.