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pENTR R4 ATCC LacZ GCTT
(Plasmid #173668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221 P4r-P3r
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 3462
  • Modifications to backbone
    addition of a LacZ cassette flanked by BsaI sites
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer M13F (after cloning! present twice before cloning)
  • 3′ sequencing primer pDONR-RP, M13R (after cloning! present twice before cloning)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Simple kanamycin resistant backbone for Golden Gate Cloning assembly using two BsaI sites flanking a LacZ cassette fro blue/white selection

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR R4 ATCC LacZ GCTT was a gift from Eric Marois (Addgene plasmid # 173668 ; http://n2t.net/addgene:173668 ; RRID:Addgene_173668)

  • For your References section:

    A population modification gene drive targeting both Saglin and Lipophorin impairs Plasmodium transmission in Anopheles mosquitoes. Green EI, Jaouen E, Klug D, Proveti Olmo R, Gautier A, Blandin S, Marois E. Elife. 2023 Dec 5;12:e93142. doi: 10.7554/eLife.93142. 10.7554/eLife.93142 PubMed 38051195
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