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ppBAalbNixE1E2E3E4 PUb-DsRed
(Plasmid #173665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221 P4r-P3r
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 8700
  • Modifications to backbone
    addition of the Nix gene under control of its own promoter and a SV40 terminator, addition of a PUb-DsRed-SV40 marker gene
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Nix masculinisation gene
  • Species
    Synthetic; Aedes albopictus
  • Insert Size (bp)
    3800
  • Mutation
    Nix isoforms with 4 exons

Gene/Insert 2

  • Gene/Insert name
    DsRed
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid sequence spanning Nix was assembled from database sequence. The actual sequence in the plasmid may contain differences due to natural polymorphism, especially in the Nix promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ppBAalbNixE1E2E3E4 PUb-DsRed was a gift from Eric Marois (Addgene plasmid # 173665 ; http://n2t.net/addgene:173665 ; RRID:Addgene_173665)

  • For your References section:

    Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus. Lutrat C, Olmo RP, Baldet T, Bouyer J, Marois E. Commun Biol. 2022 Mar 7;5(1):210. doi: 10.1038/s42003-022-03165-7. 10.1038/s42003-022-03165-7 PubMed 35256751
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