pEYK3.1
(Plasmid
#17366)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEYK3.1
- Backbone size w/o insert (bp) 3422
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Vector typeRetroviral
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Selectable markersBleomycin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
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Insert Size (bp)719
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that bacteria with this plasmid grow more slowly than normal.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEYK3.1 was a gift from George Daley (Addgene plasmid # 17366 ; http://n2t.net/addgene:17366 ; RRID:Addgene_17366) -
For your References section:
Novel retroviral vectors to facilitate expression screens in mammalian cells. Koh EY, Chen T, Daley GQ. Nucleic Acids Res. 2002 Dec 15. 30(24):e142. 10.1093/nar/gnf142 PubMed 12490733
Map uploaded by the depositor.
