DPY-pNCS
(Plasmid
#17365)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322 modified to include SV40 origin
- Backbone size w/o insert (bp) 2000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMMLV proviral DNA
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SpeciesMoloney murine leukemia virus
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Insert Size (bp)8000
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Mutationdeletion that removes 5 amino acids (DPPPY) from p12 region
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNCS contains an infectious copy of M-MuLV proviral DNA and carries a simian virus 40 replication origin to allow high-level expression in 293T cells. DPY-pNCS is similar to the wild-type parent but contains a deletion that removes 5 amino acids (DPPPY) from p12 region.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DPY-pNCS was a gift from Stephen Goff (Addgene plasmid # 17365 ; http://n2t.net/addgene:17365 ; RRID:Addgene_17365) -
For your References section:
Infectivity of Moloney murine leukemia virus defective in late assembly events is restored by late assembly domains of other retroviruses. Yuan B, Campbell S, Bacharach E, Rein A, Goff SP. J Virol. 2000 Aug . 74(16):7250-60. 10.1128/JVI.74.16.7250-7260.2000 PubMed 10906179
Map uploaded by the depositor.
