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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322 modified to include SV40 origin
- Backbone size w/o insert (bp) 2000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMMLV proviral DNA
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SpeciesMoloney murine leukemia virus
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Insert Size (bp)8000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid pNCA contains an infectious copy of MMLV proviral DNA; pNCS is a version of pNCA that carries an SV40 replication origin in the vector to allow high-level expression in 293T cells.
Sizes of insert/vector may be off depending on where the SV40 origin was inserted.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNCS was a gift from Stephen Goff (Addgene plasmid # 17362 ; http://n2t.net/addgene:17362 ; RRID:Addgene_17362) -
For your References section:
Phosphorylated serine residues and an arginine-rich domain of the moloney murine leukemia virus p12 protein are required for early events of viral infection. Yueh A, Goff SP. J Virol. 2003 Feb . 77(3):1820-9. 10.1128/JVI.77.3.1820-1829.2003 PubMed 12525616
Map uploaded by the depositor.
