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Addgene

pcDNA3/HA-SUMO3 (Sentrin 2)
(Plasmid #17361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17361 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3/HA
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO3
  • Alt name
    Sentrin2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SUMO3 (a.k.a. SMT3A, SMT3H1, SUMO-3, Smt3B)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/HA-SUMO3 (Sentrin 2) was a gift from Edward Yeh (Addgene plasmid # 17361 ; http://n2t.net/addgene:17361 ; RRID:Addgene_17361)

  • For your References section:

    Covalent modification of PML by the sentrin family of ubiquitin-like proteins. Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET. J Biol Chem. 1998 Feb 6. 273(6):3117-20. 10.1074/jbc.273.6.3117 PubMed 9452416
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