-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 17358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFlag-CMV
-
Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSENP1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1930
-
MutationR630L, K631M
-
Entrez GeneSENP1 (a.k.a. SuPr-2)
-
Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Flag-SENP1m was a gift from Edward Yeh (Addgene plasmid # 17358 ; http://n2t.net/addgene:17358 ; RRID:Addgene_17358) -
For your References section:
SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cheng J, Kang X, Zhang S, Yeh ET. Cell. 2007 Nov 2. 131(3):584-95. 10.1016/j.cell.2007.08.045 PubMed 17981124