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Addgene

Flag-SENP1
(Plasmid #17357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SENP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1930
  • Entrez Gene
    SENP1 (a.k.a. SuPr-2)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

I193F and T207A mutations are not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-SENP1 was a gift from Edward Yeh (Addgene plasmid # 17357 ; http://n2t.net/addgene:17357 ; RRID:Addgene_17357)

  • For your References section:

    SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cheng J, Kang X, Zhang S, Yeh ET. Cell. 2007 Nov 2. 131(3):584-95. 10.1016/j.cell.2007.08.045 PubMed 17981124
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