pCYB-TnsA
(Plasmid
#17356)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCYB2
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 6800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTnsA
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SpeciesE. coli transposon Tn7
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Insert Size (bp)900
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Entrez GenetnsA (a.k.a. R721_29)
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Tag
/ Fusion Protein
- Intein-Chitin binding domain (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCYB-TnsA was a gift from Nancy Craig (Addgene plasmid # 17356 ; http://n2t.net/addgene:17356 ; RRID:Addgene_17356) -
For your References section:
Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Hickman AB, Li Y, Mathew SV, May EW, Craig NL, Dyda F. Mol Cell. 2000 Jun . 5(6):1025-34. 10.1016/S1097-2765(00)80267-1 PubMed 10911996