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pFLAG-4EHP-mISG15
(Plasmid #17355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4EHP-mISG15
  • Alt name
    ISG15
  • Alt name
    4EHP
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    EIF4E2 (a.k.a. 4E-LP, 4EHP, EIF4EL3, IF4e, h4EHP)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pFLAG-4EHP-mISG15 expresses a FLAG tagged human 4EHP-mISG15(LRLRGG) fusion protein. STOP codon of 4EHP was removed by PCR. BamHI-mISG15-XbaI, which was made by PCR, was ligated to pFLAG-4EHP(stopless)/BamHI/XbaI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-4EHP-mISG15 was a gift from Dong-Er Zhang (Addgene plasmid # 17355 ; http://n2t.net/addgene:17355 ; RRID:Addgene_17355)

  • For your References section:

    ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Okumura F, Zou W, Zhang DE. Genes Dev. 2007 Feb 1. 21(3):255-60. 10.1101/gad.1521607 PubMed 17289916
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