pPiggyBacATCC LacZ GCTT loxAttP
(Plasmid #173496)

• Purpose: (Empty Backbone) piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies
• Depositing Lab: Eric Marois
• Publication: Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 10.1038/s42003-022-03165-7.

Backbone

• Vector backbone: pDONR221 P4r-P3r
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 2200
• Modifications to backbone: addition of piggyBac transposon 5' and 3' terminal repeats flanking: - one attP docking site from phage PhiC31 - a loxP cassette encompassing LacZ (for blue white selection), LacZ is flanked by two BsaI sites for Golden Gate Cloning.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Resource Information

• Supplemental Documents:
  • 905 pENTRpiggyBac lox AttP ATCC GCTT.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Golden Gate assemble your genes of interest using the type IIs BsaI (or Eco31I) restriction enzyme, which generates a
TAGG-5' overhang on one side, and a 5'-GCTT overhang on the other side of the vector. In our protocols these overhangs are termed: ATCC, GCTT (only considering the upper DNA strand for simplicity).