pET-Sfp R4-4 mutant
(Plasmid
#173491)
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PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173491 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSfp-R44
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Insert Size (bp)696
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Tag
/ Fusion Protein
- His tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-Sfp R4-4 mutant was a gift from Jun Yin (Addgene plasmid # 173491 ; http://n2t.net/addgene:173491 ; RRID:Addgene_173491) -
For your References section:
Catalytic turnover-based phage selection for engineering the substrate specificity of Sfp phosphopantetheinyl transferase. Sunbul M, Marshall NJ, Zou Y, Zhang K, Yin J. J Mol Biol. 2009 Apr 10;387(4):883-98. doi: 10.1016/j.jmb.2009.02.010. 10.1016/j.jmb.2009.02.010 PubMed 19340948
