pJK03_Rho_basal_DsRed
(Plasmid #173490)

• Purpose: Vector backbone used to clone MPRA libraries
• Depositing Lab: Barak Cohen
• Publication: White et al Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11952-7. doi: 10.1073/pnas.1307449110. Epub 2013 Jul 1.

Backbone

• Vector backbone: pCAGGS
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 3200
• Total vector size (bp): 3928
• Modifications to backbone: none
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bovine Rhodopsin promoter driving DsRed fluorescent protein
• Insert Size (bp): 847
• Promoter: Bovine Rhodopsin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: EagI (unknown if destroyed)
• 5′ sequencing primer: GATTCAGCCGGGAGCT
• 3′ sequencing primer: CTACAGGAACAGGTGGTGG

Resource Information

• Supplemental Documents:
  • pJK03_Rho_basal_MCS.gb
• A portion of this plasmid was derived from a plasmid made by: Cloning was originally performed by co-author Joseph Corbo, as described in Hsiau, et al, PLoS One 2007 Jul 25;2(7):e643. doi: 10.1371/journal.pone.0000643.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry