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pJK01_Rho_minprox_DsREd
(Plasmid #173489)

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Item Catalog # Description Quantity Price (USD)
Plasmid 173489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4015
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Rhodopsin promoter driving DsRed reporter gene
  • gRNA/shRNA sequence
    ATGGCCTCCTCCGAGAACGTCATCACCGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCACCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCCACAACACCGTGAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCCAGTACGGCTCCAAGGTGTACGTGAAGCACCCCGCCGACATCCCCGACTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGCGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCTGCTTCATCTACAAGGTGAAGTTCATCGGCGTGAACTTCCCCTCCGACGGCCCCGTGATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGACCCACAAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGTCCATCTACATGGCCAAGAAGCCCGTGCAGCTGCCCGGCTACTACTACGTGGACGCCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGCGCACCGAGGGCCGCCACCACCTGTTCCTGTA
  • Species
    Synthetic
  • Promoter Mouse Rhodopsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site EagI (unknown if destroyed)
  • 5′ sequencing primer CTAGAATGTCACCTTGGCCCC
  • 3′ sequencing primer CCACCACCTGTTCCTGTAG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This construct was originally cloned by co-author Joseph Corbo as described in Hsiau, et al. It was further modified as described in Kwasnieski, et al. Proc Natl Acad Sci U S A 2012 Nov 20;109(47):19498-503. doi: 10.1073/pnas.1210678109.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK01_Rho_minprox_DsREd was a gift from Barak Cohen (Addgene plasmid # 173489 ; http://n2t.net/addgene:173489 ; RRID:Addgene_173489)

  • For your References section:

    Massively parallel in vivo enhancer assay reveals that highly local features determine the cis-regulatory function of ChIP-seq peaks. White MA, Myers CA, Corbo JC, Cohen BA. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11952-7. doi: 10.1073/pnas.1307449110. Epub 2013 Jul 1. 10.1073/pnas.1307449110 PubMed 23818646
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