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PurposeExpresses spNW100 in Bl21
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namespNW100
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SpeciesSynthetic
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Insert Size (bp)-5
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MutationP1049L- please see depositor comments
- Promoter T7
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Tag
/ Fusion Protein
- Histag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer t7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms P1049L mutation in the insert does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
spNW100 was a gift from Huan Bao (Addgene plasmid # 173488 ; http://n2t.net/addgene:173488 ; RRID:Addgene_173488) -
For your References section:
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag. Zhang S, Ren Q, Novick SJ, Strutzenberg TS, Griffin PR, Bao H. Nat Commun. 2021 Sep 14;12(1):5451. doi: 10.1038/s41467-021-25737-7. 10.1038/s41467-021-25737-7 PubMed 34521837
