Skip to main content
Addgene

spNW25
(Plasmid #173484)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173484 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pet28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spNW25
  • Species
    Synthetic
  • Insert Size (bp)
    -5
  • Tag / Fusion Protein
    • Histag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    spNW25 was a gift from Huan Bao (Addgene plasmid # 173484 ; http://n2t.net/addgene:173484 ; RRID:Addgene_173484)
  • For your References section:

    One-step construction of circularized nanodiscs using SpyCatcher-SpyTag. Zhang S, Ren Q, Novick SJ, Strutzenberg TS, Griffin PR, Bao H. Nat Commun. 2021 Sep 14;12(1):5451. doi: 10.1038/s41467-021-25737-7. 10.1038/s41467-021-25737-7 PubMed 34521837
Commonly requested with: