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Addgene

pCMV-HA-PGRMC1-21-195
(Plasmid #173473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-HA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PGRMC1
  • Species
    H. sapiens (human)
  • Mutation
    Deleted for amino acids 1-20
  • Entrez Gene
    PGRMC1 (a.k.a. Dap1, HPR6.6, IZA, MPR)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-PGRMC1-21-195 was a gift from Bill Tsai (Addgene plasmid # 173473 ; http://n2t.net/addgene:173473 ; RRID:Addgene_173473)

  • For your References section:

    PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones. Chen YJ, Knupp J, Arunagiri A, Haataja L, Arvan P, Tsai B. Nat Commun. 2021 Oct 13;12(1):5991. doi: 10.1038/s41467-021-26225-8. 10.1038/s41467-021-26225-8 PubMed 34645803
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