pCMV-FLAG-PGRMC-Hybrid1
(Plasmid
#173469)
-
PurposeN-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 173469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV-FLAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-FLAG-PGRMC-Hybrid1 was a gift from Bill Tsai (Addgene plasmid # 173469 ; http://n2t.net/addgene:173469 ; RRID:Addgene_173469) -
For your References section:
PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones. Chen YJ, Knupp J, Arunagiri A, Haataja L, Arvan P, Tsai B. Nat Commun. 2021 Oct 13;12(1):5991. doi: 10.1038/s41467-021-26225-8. 10.1038/s41467-021-26225-8 PubMed 34645803