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pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
(Plasmid #173458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS41K-CEN ARS kanMX (type II donor template)
  • Backbone size w/o insert (bp) 6711
  • Total vector size (bp) 8166
  • Modifications to backbone
    Vector pRS41K-CEN ARS kanMX (type II donor template) (pMaM484) was digested with BamHI and SpeI and gel-purified. Insert mCherry-sfGFP was PCR amplified from pFA6a-mCherry-sfGFP-kanMX (pMaM17) and gel-purified. Vector and insert were assembled by Gibson and transformed into DH5α E. coli.
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-sfGFP
  • Insert Size (bp)
    1461
  • Promoter no promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttaccctgttatccctac
  • 3′ sequencing primer ttaaaacctaagagtcac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002) was a gift from Anton Khmelinskii (Addgene plasmid # 173458 ; http://n2t.net/addgene:173458 ; RRID:Addgene_173458)

  • For your References section:

    High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers. Fung JJ, Blocher-Juarez K, Khmelinskii A. Methods Mol Biol. 2022;2378:85-100. doi: 10.1007/978-1-0716-1732-8_6. 10.1007/978-1-0716-1732-8_6 PubMed 34985695
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