pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)
(Plasmid #173455)

• Purpose: Contains mScarlet-mNeonGreen tandem fluorescent protein timer
• Depositing Lab: Anton Khmelinskii
• Publication: Fung et al Methods Mol Biol. 2022;2378:85-100. doi: 10.1007/978-1-0716-1732-8_6.

Backbone

• Vector backbone: pFA6a-mCherry-sfGFP-kanMX (pMaM17)
• Backbone size w/o insert (bp): 5614
• Total vector size (bp): 5596
• Modifications to backbone: Plasmid pFA6a-mCherry-sfGFP-kanMX (pMaM17) was digested with SalI-HF and BamHI-HF to cut out the fluorescent proteins; the 4150bp fragment was gel-purified. mScarlet-mNeonGreen insert was amplified by PCR from plasmid pRS423-mScarlet-mNeonGreen-CYC1ter (pKBJ004.1). Digested-vector and amplified-insert were assembled by Gibson and transformed into E. coli Dh5a.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mScarlet-mNeonGreen
• Insert Size (bp): 1440
• Promoter: no promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: agagtgcaccatatggac
• 3′ sequencing primer: ctgagaaagcaacctgac

Resource Information

• Supplemental Documents:
  • pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry