pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
(Plasmid #173453)

• Purpose: Contains mCherry-mNeonGreen tandem fluorescent protein timer
• Depositing Lab: Anton Khmelinskii
• Publication: Fung et al Methods Mol Biol. 2022;2378:85-100. doi: 10.1007/978-1-0716-1732-8_6.

Backbone

• Vector backbone: pFA6a-mCherry-sfGFP-kanMX (pMaM17)
• Backbone size w/o insert (bp): 5614
• Total vector size (bp): 5608
• Modifications to backbone: Plasmid pFA6a-mCherry-sfGFP-kanMX (pMaM17) was digested with SalI-HF and BamHI-HF to cut out the fluorescent proteins; the 4150bp fragment was gel-purified. mCherry-mNeonGreen insert was amplified by PCR from plasmid pRS423-mCherry-mNeonGreen-CYC1ter (pKBJ002.1). Digested-vector and PCR-insert were assembled by Gibson and transformed into E. coli Dh5a.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-mNeonGreen
• Insert Size (bp): 1452
• Promoter: no promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: agagtgcaccatatggac
• 3′ sequencing primer: ctgagaaagcaacctgac

Resource Information

• Supplemental Documents:
  • pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry