pFA6a-mCherry-sfGFP-kanMX (pMaM17)
(Plasmid #173452)

• Purpose: Contains mCherry-sfGFP tandem fluorescent protein timer
• Depositing Lab: Anton Khmelinskii
• Publication: Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281.

Backbone

• Vector backbone: pFA6a-kanMX
• Backbone size w/o insert (bp): 3938
• Total vector size (bp): 5614
• Modifications to backbone: Backbone was linearized before the KanMx marker and mCherry-sfGFP-ADH1ter was introduced.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-sfGFP-ADH1ter
• Insert Size (bp): 1703
• Promoter: no promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: agagtgcaccatatggac
• 3′ sequencing primer: cacatcatgcccctgagc

Resource Information

• Supplemental Documents:
  • pFA6a-mCherry-sfGFP-kanMX (pMaM17).gbk
• A portion of this plasmid was derived from a plasmid made by: The plasmid was constructed and published in Khmelinskii et al. 2012 Nat Biotechnol https://www.ncbi.nlm.nih.gov/pubmed/22729030. But it was not deposited in Addgene before.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Khmelinskii et al. 2012 Nat Biotechnol https://www.ncbi.nlm.nih.gov/pubmed/22729030