AP-1 mu1-GFP
(Plasmid
#173432)
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PurposeExpression of AP-1 Mu1 subunit with a terminal GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAP1M1
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Alt nameAP-1M1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1269
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Entrez GeneAp1m1 (a.k.a. AP47, Adtm1A, Cltnm, mu1A)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer GFP REV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AP-1 mu1-GFP was a gift from Juan Bonifacino (Addgene plasmid # 173432 ; http://n2t.net/addgene:173432 ; RRID:Addgene_173432) -
For your References section:
The adaptor protein-1 mu1B subunit expands the repertoire of basolateral sorting signal recognition in epithelial cells. Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino JS. Dev Cell. 2013 Nov 11;27(3):353-66. doi: 10.1016/j.devcel.2013.10.006. 10.1016/j.devcel.2013.10.006 PubMed 24229647
