Skip to main content
Addgene

2XSmBiT-SARS-CoV-2 S1
(Plasmid #173430)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    phCMV3
  • Backbone size w/o insert (bp) 4200
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2XSmBiT SARS-CoV-2 S1-IgG1 Fc
  • Alt name
    fusion protein of SmbiT, SARS-CoV-2 Spike S1 region and human IgG1 Fc
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    3000
  • Mutation
    furin cleavage site RRAR to GSAS
  • GenBank ID
    MN908947
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • human IgG1 Fc (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2XSmBiT-SARS-CoV-2 S1 was a gift from David Nemazee (Addgene plasmid # 173430 ; http://n2t.net/addgene:173430 ; RRID:Addgene_173430)

  • For your References section:

    A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs. Huang D, Tran JT, Peng L, Yang L, Suhandynata RT, Hoffman MA, Zhao F, Song G, He WT, Limbo O, Callaghan S, Landais E, Andrabi R, Sok D, Jardine JG, Burton DR, Voss JE, Fitzgerald RL, Nemazee D. J Immunol. 2021 Jul 1;207(1):344-351. doi: 10.4049/jimmunol.2100155. Epub 2021 Jun 28. 10.4049/jimmunol.2100155 PubMed 34183368
Related items:
Commonly requested with: