RVD-GA4
(Plasmid
#173335)
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PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTetR T vector
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNN-NI dimer RVD at Position4
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SpeciesSynthetic
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Mutationno
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RVD is inserted into TA site of TetR T vector
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RVD-GA4 was a gift from Bin Shen (Addgene plasmid # 173335 ; http://n2t.net/addgene:173335 ; RRID:Addgene_173335) -
For your References section:
Precision modeling of mitochondrial diseases in zebrafish via DdCBE-mediated mtDNA base editing. Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, Shen B. Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9. 10.1038/s41421-021-00307-9 PubMed 34480028