Jag1ICD-HA pBacPAK8-3
(Plasmid
#17331)
-
Depositing Labs
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBacPAK8
-
Backbone manufacturerConaway Lab
- Backbone size w/o insert (bp) 5500
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameJagged1
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)381
-
GenBank IDNM_019147
-
Entrez GeneJag1
-
Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Bac 1
- 3′ sequencing primer Bac 2 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Jag1ICD-HA pBacPAK8-3 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 17331 ; http://n2t.net/addgene:17331 ; RRID:Addgene_17331) -
For your References section:
Neuralized-like1 targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1. Koutelou E, Sato S, Tomomori-Sato C, Florens L, Swanson SK, Washburn MP, Kokkinaki M, Conaway RC, Conaway JW, Moschonas NK. J Biol Chem. 2007 Dec 12. ():. 10.1074/jbc.M706974200 PubMed 18077452