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Addgene

pUC8-SZ
(Plasmid #17330)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 2700
  • Vector type
    for maintenance

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    streptavidin
  • Species
    Streptomyces avidinii
  • Insert Size (bp)
    2000
  • GenBank ID
    X03591

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 forward20
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was cloned in Charles Cantor's lab in Columbia University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 2kb fragment shown in Fig 2A of PubMed 3951999 was cloned into pUC-8. The streptavidin gene is roughly 500 bp away from the M13-forward20 primer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC8-SZ was a gift from Takeshi Sano (Addgene plasmid # 17330 ; http://n2t.net/addgene:17330 ; RRID:Addgene_17330)

  • For your References section:

    Molecular cloning and nucleotide sequence of the streptavidin gene. Argarana CE, Kuntz ID, Birken S, Axel R, Cantor CR. Nucleic Acids Res. 1986 Feb 25. 14(4):1871-82. 10.1093/nar/14.4.1871 PubMed 3951999
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