-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 17327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET-3a
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4600
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namestreptavidin
-
Alt nameStv-13
-
SpeciesStreptomyces avidinii
-
Insert Size (bp)400
-
MutationTruncated to include only amino acids 16-133 of mature streptavidin
-
GenBank IDX03591
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTSA-13 was a gift from Takeshi Sano (Addgene plasmid # 17327 ; http://n2t.net/addgene:17327 ; RRID:Addgene_17327) -
For your References section:
Recombinant core streptavidins. A minimum-sized core streptavidin has enhanced structural stability and higher accessibility to biotinylated macromolecules. Sano T, Pandori MW, Chen X, Smith CL, Cantor CR. J Biol Chem. 1995 Nov 24. 270(47):28204-9. 10.1074/jbc.270.47.28204 PubMed 7499314