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Addgene

pTSA-13
(Plasmid #17327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    streptavidin
  • Alt name
    Stv-13
  • Species
    Streptomyces avidinii
  • Insert Size (bp)
    400
  • Mutation
    Truncated to include only amino acids 16-133 of mature streptavidin
  • GenBank ID
    X03591

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSA-13 was a gift from Takeshi Sano (Addgene plasmid # 17327 ; http://n2t.net/addgene:17327 ; RRID:Addgene_17327)

  • For your References section:

    Recombinant core streptavidins. A minimum-sized core streptavidin has enhanced structural stability and higher accessibility to biotinylated macromolecules. Sano T, Pandori MW, Chen X, Smith CL, Cantor CR. J Biol Chem. 1995 Nov 24. 270(47):28204-9. 10.1074/jbc.270.47.28204 PubMed 7499314
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