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pFGC-T7RibJ-RRvT
(Plasmid #173226)

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Item Catalog # Description Quantity Price (USD)
Plasmid 173226 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUCIDT-KAN
  • Backbone manufacturer
    IDT
  • Total vector size (bp) 4591
  • Vector type
    Bacterial Expression ; Cell-free system

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RRvT
  • Insert Size (bp)
    1460
  • Promoter T7

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC
  • 3′ sequencing primer GGTGGAAGGGCTCGGAGTTGTGGTAATCTATGTATCCTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGC-T7RibJ-RRvT was a gift from Jim Haseloff (Addgene plasmid # 173226 ; http://n2t.net/addgene:173226 ; RRID:Addgene_173226)

  • For your References section:

    Constructing Cell-Free Expression Systems for Low-Cost Access. Guzman-Chavez F, Arce A, Adhikari A, Vadhin S, Pedroza-Garcia JA, Gandini C, Ajioka JW, Molloy J, Sanchez-Nieto S, Varner JD, Federici F, Haseloff J. ACS Synth Biol. 2022 Mar 18;11(3):1114-1128. doi: 10.1021/acssynbio.1c00342. Epub 2022 Mar 8. 10.1021/acssynbio.1c00342 PubMed 35259873
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