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pM162
(Plasmid #173221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-DEST
  • Backbone size w/o insert (bp) 6589
  • Total vector size (bp) 11824
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
  • Species
    Synthetic
  • Insert Size (bp)
    2280
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer gatcgcctggagacgccatc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    MCP-mCherry-GFP11-GB1
  • Species
    Synthetic
  • Insert Size (bp)
    2316
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer ggccgcgtggataaccgtattac
  • 3′ sequencing primer agttaagaataccagtcaatctttcac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All-in-one plasmid containing mCherry and Puromycin selection markers

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM162 was a gift from Hajin Kim (Addgene plasmid # 173221 ; http://n2t.net/addgene:173221 ; RRID:Addgene_173221)

  • For your References section:

    Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore. Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H. Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. Epub 2020 Sep 4. 10.1101/gr.260018.119 PubMed 32887690
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