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Addgene

2xHA MED10 pBacPAK8
(Plasmid #17321)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MED10
  • Alt name
    Med10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Entrez Gene
    Med10 (a.k.a. C78613, AA959813, AI385605, D13Wsu50e)
  • Tag / Fusion Protein
    • 2xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer Bac 1
  • 3′ sequencing primer Bac 2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2xHA MED10 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 17321 ; http://n2t.net/addgene:17321 ; RRID:Addgene_17321)

  • For your References section:

    Neuralized-like1 targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1. Koutelou E, Sato S, Tomomori-Sato C, Florens L, Swanson SK, Washburn MP, Kokkinaki M, Conaway RC, Conaway JW, Moschonas NK. J Biol Chem. 2007 Dec 12. ():. 10.1074/jbc.M706974200 PubMed 18077452
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