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Addgene

pAAV-hSyn-Mito-HyPerGRt
(Plasmid #173198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSyn
  • Backbone size w/o insert (bp) 4268
  • Total vector size (bp) 7371
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hyper7-tdTomato
  • Alt name
    HyPerGRT
  • Species
    Synthetic
  • Insert Size (bp)
    3089
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer agctgctagcaaggatcccatcg
  • 3′ sequencing primer ctttcacaaattttgtaatccagag
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-Mito-HyPerGRt was a gift from Huiwang Ai (Addgene plasmid # 173198 ; http://n2t.net/addgene:173198 ; RRID:Addgene_173198)

  • For your References section:

    Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in Abeta42-Mediated Neurotoxicity. Li X, Zhang Y, Ai HW. ACS Sens. 2022 Feb 28. doi: 10.1021/acssensors.1c01381. 10.1021/acssensors.1c01381 PubMed 35226474
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